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Crosslinked with the highest efficiency among all 45 proteins we tested, including 10 known RBPs, were newly identified candidate RBPs (Table 1). We also found that whether or not a candidate RBP had been reported to interact physically with a known RBP was not a strong predictor of whether it could be cross-linked directly to RNA in our assay (4/5 for the potential indirect binders and 19/25 for

Hakeem akintunde Ogunmowo Real Estate Developers One key to the success of a shopping center is quick and easy automobile access. Such access is generally more available in the suburbs than in the city. A few successful shopping centers have been built in dense urban areas, often with very substantial public subsidy and often as part of an urban renewal project. However, on balance, the invention and refinement of the shopping center has favored suburb over city. Not only has the suburban sho

Hakeem akintunde Ogunmowo Real Estate Developers One key to the success of a shopping center is quick and easy automobile access. Such access is generally more available in the suburbs than in the city. A few successful shopping centers have been built in dense urban areas, often with very substantial public subsidy and often as part of an urban renewal project. However, on balance, the invention and refinement of the shopping center has favored suburb over city. Not only has the suburban sho
H a given RNA bind concurrently, sequentially, or in mutually exclusive cellular locations. In contrast, our RNA-dependent interaction data enable us to directly identify pairs of proteins that bind concurrently to one or more RNAs inGenome Researchwww.genome.orgKlass et al.processes other than mRNA polyadenylation and export (Fig. 6). We detected novel RNA-dependent interactions between Nab2 and
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Protease digest of purified proteins to reveal structured regions cross-linked to radioactively labeled, exhaustively digested RNA fragments. We then analyzed the digestion products by SDS-PAGE to find distinct bands representing ordered domains of the protein (Fontana et al. 2012). Next, we measured the radioactivity of each of these bands to determine if they were cross-linked to RNA. Protease d
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